Bioluminescence Assays and Reagents
Gold Biotechnology Inc D-Luciferin Firefly, free acid (Proven and Published™) 1 g
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Cellomics Technology LLC MV4-11/GFP-FIREFLY LUCIFERASE
MV4-11/GFP-firefly luciferase (CMV) stable cells. 2x106 cells
BPS Bioscience Inc STAT3 Reporter Kit (STAT3 Signaling Pathway) | 500 Reactions
The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
BPS Bioscience Inc RENILLA LUCIFERASE LENTIVIRUS
500ulx2 The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS
Apexbio Technology LLC EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP), 100ug (1mg/mL)
EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) will express luciferase protein once entering cells which is initially extracted from firefly Photinus pyralis . This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC EZ Cap™ Cy5 EGFP mRNA (5-moUTP), 5x1mg (1mg/mL)
EZ Cap™ Cy5 EGFP mRNA (5-moUTP) will express enhanced green fluorescent protein(EGFP) once entering cells originally isolated from the jellyfish(Aequorea victoria). You can observe the green fluorescence at 509 nm. EGFP is usually used as a reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc. Other sizes are also available. Please inqury us for quote.
Electron Microscopy Sciences CryoCapsule Perforated Petri Dish for Live Cell
For CryoCapsule correlative light and electron microscopy high pressure freezing cell.
Biotium Renilla Luciferase Assay Kit 2.0, 1000 Assays
This kit is designed to measure Renilla luciferase activity in transfected cell reporter gene assays. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. The enzyme is a a monomeric 36,000 Dalton protein that catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. This assay kit utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. The kit is suitable for plate sizes ranging from 96-well to 24-well and has reagents sufficient for 1000 assays.
Biotium Steady-Luc™ Firefly HTS Assay Kit (Lyophilized), 1000 Assays
Biotium’s Steady-Luc™ HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a single-step, homogeneous, high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates. Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity. The kit is offered in a lyophilized format for convenient room temperature shipping and -20⁰C storage. The kit has reagents sufficient for 1000 assays in a 96-well format.